The main goal of this research is studying the effect of media diplomacy on cultural exchange among countries. While considering that the accessibility to all members of the community is not possible, and we cannot prepare a list of the whole members of the population, therefore, the research population is positioning in an unlimited community scope. In the current research, numbers of 33 persons are considered as adequate sample size by using an ordered random sampling method with following the assumption of descriptive researches from survey type. Regarding that the research population is placed in an unlimited community scope, sampling method of current research is non-random and from targeted type. The methodology of this research from purpose point of view is applied and from data collecting point of view is descriptive. Researcher made questionnaire is used for collecting data. Its formal and content justifiability were obtained through available resources and experts’ opinions and its validity was determined in 0.90 through trial execution and calculation of Cronbach Alpha Coefficient. Descriptive and inferential statistical tests were used for data analysis. Findings of the research are demonstrating that the effect of media diplomacy on acculturation except the factors of “creating new culture from combination of previous culture and dominant culture in a country”, the effect of media diplomacy on culturalization except factors of “exchanging of professors and students between agent country and target country”, “legislation for fulfilling cultural relations with countries” and “execution of agreements, memorandum of understanding and cultural exchanges program of countries” and the effect of media diplomacy on presenting an optimal image in international arena is higher than the average and in other mentioned factors, the effect is in average extent.